Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Long non-coding RNA and mRNA profile analysis of metformin to reverse the pulmonary hypertension vascular remodeling induced by monocrotaline.

doi: 10.1016/j.biopha.2019.108933

Figure Lengend Snippet: Fig. 4. Signal pathway identification and transcription factor activity verification. A: Representative western blotting strips of PAs from MCT-induced PAH rats with or without metformin treatment and densitometric analysis showing Hif1α, PKG relative to β-actin; B: Representative western blotting strips of PAs from MCT- induced PAH rats with or without metformin treatment and densitometric analysis showing p-AKT relative to AKT; C: Representative western blotting strips of PAs from MCT-induced PAH rats with or without metformin treatment and densitometric analysis showing p53 relative to β-actin; D: Representative western blotting strips of PAs from MCT-induced PAH rats with or without metformin treatment and densitometric analysis showing Sp1, Est1 relative to β-actin. MCT means monocrotaline; Met means metformin. **p < 0.01. n = 3.

Article Snippet: The dilution ratio of all primary antibodies used was as follows: (PKG, catalog number: 21646-1-AP, 1:1000 dilution, proteintech), (Sp1, catalog number: 21962-1-AP, 1:2000 dilution, proteintech), (Est1, catalog number: 12522-1-AP, 1:1000 dilution, proteintech), (Hif1α, Cat. #: AF1009, 1:2000 dilution, Affinity Biosciences), (p53, catalog number: 10442-1-AP, 1:5000 dilution, proteintech), (Cyclin D1, catalog number: 26755-1-AP, 1:1000 dilution, proteintech), (β-actin, catalog number: 60008-1-1 g, 1:10000 dilution, proteintech), (AKT, catalog number: 10176-2-AP, 1:2000 dilution, proteintech), (p-AKT, # 9271, 1:1000 dilution, Cell Signaling Technology).

Techniques: Activity Assay, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Long non-coding RNA and mRNA profile analysis of metformin to reverse the pulmonary hypertension vascular remodeling induced by monocrotaline.

doi: 10.1016/j.biopha.2019.108933

Figure Lengend Snippet: Fig. 7. Mechanism analysis of NONRATT015587.2 regulation of pulmonary vascular remodeling in vitro assay and differential gene clustergram in MCT-induced PAH rats and controls, or metformin treatment. A-G: Realtime-PCR analysis of p53, Est1, Rela, Cebpa/b, Sp1, Hif1α expression in pcDNA, pcDNA- NONRATT015587.2, and pcDNA- NONRATT015587.2+metformin group. H: Representative western blotting strips of PASMCs from pcDNA-NONRATT015587.2 with or without metformin treatment and densitometric analysis showing Hif1α, p53 relative to β-actin; I: The differential expression of mRNA in the microarray analysis. Hierarchical clustering analysis is shown for 32 mRNA (fold change > 2, p < 0.05) in the control, monocrotaline and monocrotaline + metformin group using gene microarray analysis. MCT means monocrotaline; Met means metformin. **p < 0.01. n = 3.

Article Snippet: The dilution ratio of all primary antibodies used was as follows: (PKG, catalog number: 21646-1-AP, 1:1000 dilution, proteintech), (Sp1, catalog number: 21962-1-AP, 1:2000 dilution, proteintech), (Est1, catalog number: 12522-1-AP, 1:1000 dilution, proteintech), (Hif1α, Cat. #: AF1009, 1:2000 dilution, Affinity Biosciences), (p53, catalog number: 10442-1-AP, 1:5000 dilution, proteintech), (Cyclin D1, catalog number: 26755-1-AP, 1:1000 dilution, proteintech), (β-actin, catalog number: 60008-1-1 g, 1:10000 dilution, proteintech), (AKT, catalog number: 10176-2-AP, 1:2000 dilution, proteintech), (p-AKT, # 9271, 1:1000 dilution, Cell Signaling Technology).

Techniques: In Vitro, Expressing, Western Blot, Quantitative Proteomics, Microarray, Control

Journal: Nutrition & Diabetes

Article Title: Non-pharmacological interventions of intermittent fasting and pulsed radiofrequency energy (PRFE) combination therapy promote diabetic wound healing

doi: 10.1038/s41387-024-00344-9

Figure Lengend Snippet: The sequences of primer pairs used in qPCR.

Article Snippet: The primary antibody includes antibodies targeting VEGFA (1:1000, A12303, Abclone, China), COL1A2 (1: 2000, 14695-1-AP, Proteintech, China), HIF1A (1:1000, HY-P80704, MCE, USA), CGRP (1:1000, A5542, Abclone, China), P75 (1:1000, A19127, Abclone, China), α-Tubulin (1:10000, A19127, Abclone, China).

Techniques:

Journal: Nutrition & Diabetes

Article Title: Non-pharmacological interventions of intermittent fasting and pulsed radiofrequency energy (PRFE) combination therapy promote diabetic wound healing

doi: 10.1038/s41387-024-00344-9

Figure Lengend Snippet: A Representative fluorescence images and B quantification showing local ROS levels of HUVECs in the Control group, PRFE group, Fasting group, and PRFE+Fasting group. Scale bar = 100 μm. C qRT-PCR analysis of the expression levels of HIF1A target gene in HUVECs in different groups. n = 3 per group. D Representative western blotting and E relative quantitative analysis of HIF1A in HUVECs in each group. The densitometric are normalized by α-tubulin. n = 3 per group. F qRT-PCR analysis of the expression levels of HIF1A target gene in mice wounds in each group. n = 3 per group. G Representative western blotting and H relative quantitative analysis of HIF1A in whole-cell protein extracts from mice wounds in different treatment groups. The densitometric are normalized by α-tubulin. n = 3 per group. I Representative immunohistochemical staining images and J the relative IOD of HIF1A expressions of mice wounds in different treatment groups. Scale bar = 100 μm. n = 3 per group. B , C , E , F , H , J One-way ANOVA combined with Bonferroni post hoc test. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The primary antibody includes antibodies targeting VEGFA (1:1000, A12303, Abclone, China), COL1A2 (1: 2000, 14695-1-AP, Proteintech, China), HIF1A (1:1000, HY-P80704, MCE, USA), CGRP (1:1000, A5542, Abclone, China), P75 (1:1000, A19127, Abclone, China), α-Tubulin (1:10000, A19127, Abclone, China).

Techniques: Fluorescence, Control, Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: Biology Direct

Article Title: Catalase inhibits tumor growth by alleviating oxidative stress and promoting the polarization of tumor-associated macrophage from M2 to M1 phenotype in non-small cell lung cancer

doi: 10.1186/s13062-026-00730-6

Figure Lengend Snippet: The xenograft nude mice model with A549 cell-burdened. ( A ) Schematic diagram of the construction of the xenograft tumor mouse model; ( B ) Images of xenograft tumors with scale bars, comparing tumor sizes between the A549-CAT-VC and A549-CAT-OE groups; ( C ) Tumor growth curve comparison between the A549-CAT-VC and A549-CAT-OE groups; ( D ) Bar chart comparing the tumor volumes of mice in the A549-CAT-VC and A549-CAT-OE groups; ( E-F ) Flow cytometry proportion plot ( E ) and bar chart ( F ) of M1 macrophages in the A549-CAT-VC and A549-CAT-OE groups; ( G-H ) Flow cytometry proportion plot ( G ) and bar chart ( H ) of M2 macrophages in the A549-CAT-VC and A549-CAT-OE groups; ( I ) Schematic diagram of the immunohistochemistry; ( J ) CAT histochemical staining images of tumors in the A549-CAT-VC and A549-CAT-OE groups; ( K ) HIF1α histochemical staining images of tumors in the A549-CAT-VC and A549-CAT-OE groups

Article Snippet: After blocking with 5% (w/v) non-fat milk for 1 h at 25 °C, the membranes were incubated overnight at 4 °C with primary antibodies against Catalase (21260-1-AP, 1:1000, Proteintech, China), GAPDH (60004-1-Ig, 1:3000, Proteintech, China), beta-actin (4970 S, 1:1000, CST, USA), and HIF1-alpha (80933-1-RR, 1:1000, Proteintech, China).

Techniques: Comparison, Flow Cytometry, Immunohistochemistry, Staining

Journal: Cell Death & Disease

Article Title: p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis

doi: 10.1038/s41419-020-02781-7

Figure Lengend Snippet: a – f Relative mRNA expression of Δ113p53 ( a ), gpx1a ( b ), sesn2 ( c ), hif1al2 ( d ), jak2a ( e ) and pim2 ( f ) in the Δ113p53 +/+ and Δ113p53 M/M hearts at sham and 14 dpa. The total RNA was extracted from a pool of at least 10 hearts in each group. g Western blot was performed to analyse the induction of zebrafish Hif1α in different samples as indicated. The Δ113p53 +/+ and Δ113p53 M/M mutant zebrafish with heart resection were treated with DPI from 3 to 7 dpa. Total protein was isolated from four hearts/treatment at 7 dpa and subjected to western blot analysis. Gapdh was used as the protein loading control. The experiments were repeated independently for at least three times with similar results. Statistical analysis was performed on relevant data using Student’s two-tailed t test in GraphPad Prism 5. The p values were represented by n.s. and asterisks. n.s., p > 0.05. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: A human HIF1α antibody (BOSTER, A00013-1) was used to detect zebrafish Hif1a.

Techniques: Expressing, Western Blot, Mutagenesis, Isolation, Control, Two Tailed Test

Journal: Journal of Inflammation Research

Article Title: Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis

doi: 10.2147/JIR.S254717

Figure Lengend Snippet: Relative quantification of NF-kB p65, TLR4, and TLR2 protein expression levels. ( A ) Western blot analysis for NF-kB p65, TLR4, and TLR2 relative expression from groups indicated. ( B ) summary graph for relative expression of NF-kB (n=8 per group). ( C ) summary graph for relative expression of TLR4 (n=8 per group). ( D ) summary graph for relative expression of TLR2 (n=8 per group). All values are represented as mean ± SEM. * p<0.05; ** p<0.01; and *** p<0.001 versus the vehicle control group.

Article Snippet: Samples were subsequently treated with anti β-actin antibody (BM3873, Boster Biological Technology, China), NF-kB p65 monoclonal antibody (A10609, Abclonal, USA.), TLR4 antibody (BA1717, Boster Biological Technology, China), or TLR2 antibody (BM4001, Boster Biological Technology, China), and were incubated overnight at 4°C.

Techniques: Quantitative Proteomics, Expressing, Western Blot, Control